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PATL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PATL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PATL2 antibodies →
$504.00each
Catalog Number: LS-G126530-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PATL2 (NM_001145112), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HPat1a | PAT1-like protein 2 | Pat1a | PATL2 | Protein PAT1 homolog a | Protein PAT1 homolog 2
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNCLEGPGKT CGPLASEEEL VSACQLEKEE ENEGEEEEEE EDEEDLDPDL DPDLEEEEND
61LGDPAVLGAV HNTQRALLSS PGVKAPGMLG MSLASLHFLW QTLDYLSPIP FWPTFPSTSS
121PAQHFGPRLP SPDPTLFCSL LTSWPPRFSH LTQLHPRHQR ILQQQQHSQT PSPPAKKPWS
181QQPDPYANLM TRKEKDWVIK VQMVQLQSAK PRLDDYYYQE YYQKLEKKQA DEELLGRRNR
241VESLKLVTPY IPKAEAYESV VRIEGSLGQV AVSTCFSPRR AIDAVPHGTQ EQDIEAASSQ
301RLRVLYRIEK MFLQLLEIEE GWKYRPPPPC FSEQQSNQVE KLFQTLKTQE QNNLEEAADG
361FLQVLSVRKG KALVARLLPF LPQDQAVTIL LAITHHLPLL VRRDVADQAL QMLFKPLGKC
421ISHLTLHELL QGLQGLTLLP PGSSERPVTV VLQNQFGISL LYALLSHGEQ LVSLHSSLEE
481PNSDHTAWTD MVVLIAWEIA QMPTASLAEP LAFPSNLLPL FCHHVDKQLV QQLEARMEFA
541WIY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.