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PATL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PATL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106063-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PATL1 (NM_152716), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HPat1b | PAT1-like protein 1 | PATL1 | Protein PAT1 homolog b | Protein PAT1 homolog 1 | Pat1b
Origin Species
Human
NCBI GenBank
NM_152716
SwissProt
Q86TB9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPTVSVLEYA LPQRPPQGPE DDRDLSERAL PRRSTSPIIG SPPVRAVPIG TPPKQMAVPS
61FTQQILCPKP VHVRPPMPPR YPAPYGERMS PNQLCSVPNS SLLGHPFPPS VPPVLSPLQR
121AQLLGGAQLQ PGRMSPSQFA RVPGFVGSPL AAMNPKLLQG RVGQMLPPAP GFRAFFSAPP
181SATPPPQQHP PGPGPHLQNL RSQAPMFRPD TTHLHPQHRR LLHQRQQQNR SQHRNLNGAG
241DRGSHRSSHQ DHLRKDPYAN LMLQREKDWV SKIQMMQLQS TDPYLDDFYY QNYFEKLEKL
301SAAEEIQGDG PKKERTKLIT PQVAKLEHAY KPVQFEGSLG KLTVSSVNNP RKMIDAVVTS
361RSEDDETKEK QVRDKRRKTL VIIEKTYSLL LDVEDYERRY LLSLEEERPA LMDDRKHKIC
421SMYDNLRGKL PGQERPSDDH FVQIMCIRKG KRMVARILPF LSTEQAADIL MTTARNLPFL
481IKKDAQDEVL PCLLSPFSLL LYHLPSVSIT SLLRQLMNLP QSAATPALSN PHLTAVLQNK
541FGLSLLLILL SRGEDLQSSD PATESTQNNQ WTEVMFMATR ELLRIPQAAL AKPISIPTNL
601VSLFSRYVDR QKLNLLETKL QLVQGIR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

627 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.