LSBio
PASD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PASD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PASD1 antibodies →
$504.00each
Catalog Number: LS-G102440-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PASD1 (NM_173493), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 63 | CT63 | PAS domain containing 1 | OX-TES-1 | OXTES1 | PASD1
Origin Species
Human
SwissProt
Q8IV76

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKMRGEKRRD KVNPKSSQRK LNWIPSFPTY DYFNQVTLQL LDGFMITLST DGVIICVAEN
61ISSLLGHLPA EIVGKKLLSL LPDEEKDEVY QKIILKFPLL NSETHIEFCC HLKRGNVEHG
121DSSAYENVKF IVNVRDICNE FPVVFSGLFS SHLCADFAAC VPQEDRLYLV GNVCILRTQL
181LQQLYTSKAV SDEAVLTQDS DEEPFVGELS SSQGQRGHTS MKAVYVEPAA AAAAAAISDD
241QIDIAEVEQY GPQENVHMFV DSDSTYCSST VFLDTMPESP ALSLQDFRGE PEVNPLYRAD
301PVDLEFSVDQ VDSVDQEGPM DQQDPENPVA PLDQAGLMDP VDPEDSVDLG AAGASAQPLQ
361PSSPVAYDII SQELELMKKL KEQLEERTWL LHDAIQNQQN ALELMMDHLQ KQPNTLRHVV
421IPDLQSSEAV PKKQQKQHAG QVKRPLPHPK DVKCFCGLSL SNSLKNTGEL QEPCVAFNQQ
481QLVQQEQHLK EQQRQLREQL QQLREQRKVQ KQKKMQEKKK LQEQKMQEKK KLQEQRRQKK
541KKLQERKKWQ GQMLQKEPEE EQQKQQLQEQ PLKHNVIVGN ERVQICLQNP RDVSVPLCNH
601PVRFLQAQPI VPVQRAAEQQ PSGFYQDENC GQQEDESQSF YPEAYQGPPV NQLPLIDTSN
661SEAISSSSIP QFPITSDSTI STLETPQDYI RLWQELSDSL GPVVQVNTWS CDEQGTLHGQ
721PTYHQVQVSE VGVEGPPDPQ AFQGPAAYQP DQMRSAEQTR LMPAEQRDSN KPC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

773 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.