LSBio
PARP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PARP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PARP8 antibodies →
$725.00each
Catalog Number: LS-G109894-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PARP8 (NM_024615), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARTD16 | Poly [ADP-ribose] polymerase 8 | PARP-8 | PARP8 | PART16
Origin Species
Human
NCBI GenBank
NM_024615
SwissProt
Q8N3A8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MGMCSRQERI QKDIDVVIQK SRAEKDCLFA DFRYSDSTFT FTYVGGPRSV SYSVHVSEDY
61PDNTYVSSSE NDEDVLVTTE PIPVIFHRIA TELRKTNDIN CCLSIKSKLQ KENGEESRQN
121STVEEDSEGD NDSEEFYYGG QVNYDGELHK HPQLEADLSA VREIYGPHAV SLREYGAIDD
181VDIDLHIDVS FLDEEIAVAW EVIRTEPIIV RLHCSLTQYL NGPVPTVDVF QISTKERFGL
241GHQLKKIMQT FVTQQWKQSK EKSNCLHNKK LSEKKVKSPL HLFSTLRRSP SYPPPGCGKS
301KSKLKSEQDG ISKTHKLLRR TCSSTVKTDD VCVTKSHRTF GRSLSSDPRA EQAMTAIKSH
361KLLNRPCPAA VKSEECLTLK SHRLLTRSCS GDPRCEHNTN LKPHKLLSRS YSSNLRMEEL
421YGLKNHKLLS KSYSSAPKSS KTELFKEPNA EGRRLSLTSG LIGILTPSSS SSSQLAPNGA
481KCIPVRDRGF LVQTIEFAEQ RIPVLNEYCV VCDEPHVFQN GPMLRPTVCE RELCVFAFQT
541LGVMNEAADE IATGAQVVDL LVSMCRSALE SPRKVVIFEP YPSVVDPNDP QMLAFNPRKK
601NYDRVMKALD SITSIREMTQ APYLEIKKQM DKQDPLAHPL LQWVISSNRS HIVKLPVNRQ
661LKFMHTPHQF LLLSSPPAKE SNFRAAKKLF GSTFAFHGSH IENWHSILRN GLVVASNTRL
721QLHGAMYGSG IYLSPMSSIS FGYSGMNKKQ KVSAKDEPAS SSKSSNTSQS QKKGQQSQFL
781QSRNLKCIAL CEVITSSDLH KHGEIWVVPN TDHVCTRFFF VYEDGQVGDA NINTQEGGIH
841KEILRVIGNQ TATG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

854 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.