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PARP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PARP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101647-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PARP6 (NM_020214), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARTD17 | PARP-6-C | PARP6 | Poly [ADP-ribose] polymerase 6 | PARP-6 | PARP-6-B1 | PART17
Origin Species
Human
NCBI GenBank
NM_020214
SwissProt
Q2NL67

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDIKGQFWND DDSEGDNESE EFLYGVQGSC AADLYRHPQL DADIEAVKEI YSENSVSIRE
61YGTIDDVDID LHINISFLDE EVSTAWKVLR TEPIVLRLRF SLSQYLDGPE PSIEVFQPSN
121KEGFGLGLQL KKILGMFTSQ QWKHLSNDFL KTQQEKRHSW FKASGTIKKF RAGLSIFSPI
181PKSPSFPIIQ DSMLKGKLGV PELRVGRLMN RSISCTMKNP KVEVFGYPPS PQAGLLCPQH
241VGLPPPARTS PLVSGHCKNI PTLEYGFLVQ IMKYAEQRIP TLNEYCVVCD EQHVFQNGSM
301LKPAVCTREL CVFSFYTLGV MSGAAEEVAT GAEVVDLLVA MCRAALESPR KSIIFEPYPS
361VVDPTDPKTL AFNPKKKNYE RLQKALDSVM SIREMTQGSY LEIKKQMDKL DPLAHPLLQW
421IISSNRSHIV KLPLSRLKFM HTSHQFLLLS SPPAKEARFR TAKKLYGSTF AFHGSHIENW
481HSILRNGLVN ASYTKLQLHG AAYGKGIYLS PISSISFGYS GMGKGQHRMP SKDELVQRYN
541RMNTIPQTRS IQSRFLQSRN LNCIALCEVI TSKDLQKHGN IWVCPVSDHV CTRFFFVYED
601GQVGDANINT QDPKIQKEIM RVIGTQVYTN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

630 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.