Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Paralemmin / PALM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117893-100
Catalog Number: LS-G117893
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Paralemmin(PALM) (NM_002579), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Paralemmin | KIAA0270 | Paralemmin-1 | PALM
- Origin Species
- Human
- NCBI GenBank
- NM_002579
- SwissProt
- O75781
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVLAAETTS QQERLQAIAE KRKRQAEIEN KRRQLEDERR QLQHLKSKAL RERWLLEGTP61SSASEGDEDL RRQMQDDEQK TRLLEDSVSR LEKEIEVLER GDSAPATAKE NAAAPSPVRA121PAPSPAKEER KTEVVMNSQQ TPVGTPKDKR VSNTPLRTVD GSPMMKAAMY SVEITVEKDK181VTGETRVLSS TTLLPRQPLP LGIKVYEDET KVVHAVDGTA ENGIHPLSSS EVDELIHKAD241EVTLSEAGST AGAAETRGAV EGAARTTPSR REITGVQAQP GEATSGPPGI QPGQEPPVTM301IFMGYQNVED EAETKKVLGL QDTITAELVV IEDAAEPKEP APPNGSAAEP PTEAASREEN361QAGPEATTSD PQDLDMKKHR CKCCSIMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
387 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.