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PAPOLG Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAPOLG Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110156-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAPOLG (NM_022894), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PAP2 | PAPG | PAPOLG | Neo-PAP | Neo-poly(A) polymerase | PAP-gamma | Poly(A) polymerase gamma | SRP RNA 3-adenylating enzyme
Origin Species
Human
NCBI GenBank
NM_022894
SwissProt
Q9BWT3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MKEMSANTVL DSQRQQKHYG ITSPISLASP KEIDHIYTQK LIDAMKPFGV FEDEEELNHR
61LVVLGKLNNL VKEWISDVSE SKNLPPSVVA TVGGKIFTFG SYRLGVHTKG ADIDALCVAP
121RHVERSDFFQ SFFEKLKHQD GIRNLRAVED AFVPVIKFEF DGIEIDLVFA RLAIQTISDN
181LDLRDDSRLR SLDIRCIRSL NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML
241GFLGGVSWAM LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD
301PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI LQGKSDWSKL
361LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL VGNLERNEFI TLAHVNPQSF
421PGNKEHHKDN NYVSMWFLGI IFRRVENAES VNIDLTYDIQ SFTDTVYRQA NNINMLKEGM
481KIEATHVKKK QLHHYLPAEI LQKKKKQSLS DVNRSSGGLQ SKRLSLDSSC LDSSRDTDNG
541TPFNSPASKS DSPSVGETER NSAEPAAVIV EKPLSVPPAQ GLSIPVIGAK VDSTVKTVSP
601PTVCTIPTVV GRNVIPRITT PHNPAQGQPH LNGMSNITKT VTPKRSHSPS IDGTPKRLKD
661VEKFIRLEST FKDPRTAEER KIKSVDAIGG ESMPIPTIDT SRKKRLPSKE LPDSSSPVPA
721NNIRVIKNSI RLTLNR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

736 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.