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PAPLN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAPLN Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105346-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAPLN (NM_173462), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Papilin | PAPLN
Origin Species
Human
SwissProt
O95428

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
134.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLLLLVPLL LAPAPGSSAP KVRRQSDTWG PWSQWSPCSR TCGGGVSFRE RPCYSQRRDG
61GSSCVGPARS HRSCRTESCP DGARDFRAEQ CAEFDGAEFQ GRRYRWLPYY SAPNKCELNC
121IPKGENFYYK HREAVVDGTP CEPGKRDVCV DGSCRVVGCD HELDSSKQED KCLRCGGDGT
181TCYPVAGTFD ANDLSRAVKN VRGEYYLNGH WTIEAARALP AASTILHYER GAEGDLAPER
241LHARGPTSEP LVIELISQEP NPGVHYEYHL PLRRPSPGFS WSHGSWSDCS AECGGGHQSR
301LVFCTIDHEA YPDHMCQRQP RPADRRSCNL HPCPETKRWK AGPWAPCSAS CGGGSQSRSV
361YCISSDGAGI QEAVEEAECA GLPGKPPAIQ ACNLQRCAAW SPEPWGECSV SCGVGVRKRS
421VTCRGERGSL LHTAACSLED RPPLTEPCVH EDCPLLSDQA WHVGTWGLCS KSCSSGTRRR
481QVICAIGPPS HCGSLQHSKP VDVEPCNTQP CHLPQEVPSM QDVHTPASNP WMPLGPQESP
541ASDSRGQWWA AQEHPSARGD HRGERGDPRG DQGTHLSALG PAPSLQQPPY QQPLRSGSGP
601HDCRHSPHGC CPDGHTASLG PQWQGCPGAP CQQSRYGCCP DRVSVAEGPH HAGCTKSYGG
661DSTGGMPRSR AVASTVHNTH QPQAQQNEPS ECRGSQFGCC YDNVATAAGP LGEGCVGQPS
721HAYPVRCLLP SAHGSCADWA ARWYFVASVG QCNRFWYGGC HGNANNFASE QECMSSCQGS
781LHGPRRPQPG ASGRSTHTDG GGSSPAGEQE PSQHRTGAAV QRKPWPSGGL WRQDQQPGPG
841EAPHTQAFGE WPWGQELGSR APGLGGDAGS PAPPFHSSSY RISLAGVEPS LVQAALGQLV
901RLSCSDDTAP ESQAAWQKDG QPISSDRHRL QFDGSLIIHP LQAEDAGTYS CGSTRPGRDS
961QKIQLRIIGG DMAVLSEAEL SRFPQPRDPA QDFGQAGAAG PLGAIPSSHP QPANRLRLDQ
1021NQPRVVDASP GQRIRMTCRA EGFPPPAIEW QRDGQPVSSP RHQLQPDGSL VISRVAVEDG
1081GFYTCVAFNG QDRDQRWVQL RVLGELTISG LPPTVTVPEG DTARLLCVVA GESVNIRWSR
1141NGLPVQADGH RVHQSPDGTL LIYNLRARDE GSYTCSAYQG SQAVSRSTEV KVVSPAPTAQ
1201PRDPGRDCVD QPELANCDLI LQAQLCGNEY YSSFCCASCS RFQPHAQPIW Q
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1251 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.