Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PAOX / PAO Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102838-100
Catalog Number: LS-G102838
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAOX (NM_207128), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PAO | PAOX | Polyamine oxidase
- Origin Species
- Human
- NCBI GenBank
- AA418209
- SwissProt
- Q6QHF9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MESTGSVGEA PGGPRVLVVG GGIAGLGAAQ RLCGHSAFPH LRVLEATARA GGRIRSERCF61GGVVEVGAHW IHGPSRGNPV FQLAAEYGLL GEKELSQENQ LVETGGHVGL PSVSYASSGA121SVSLQLVAEM ATLFYGLIDQ TREFLHAAET PVPSVGEYLK KEIGQHVAGW TEDEETRKLK181LAVLNSFFNL ECCVSGTHSM DLVALAPFGE YTVLPGLDCT FSKGYQGLTN CMMAALPEDT241VVFEKPVKTI HWNGSFQEAA FPGETFPVSV ECEDGDRFPA HHVIVTVPLG FLREHLDTFF301DPPLPAEKAE AIRKIGFGTN NKIFLEFEEP FWEPDCQLIQ LVWEDTSPLE DAAPELQDAW361FRKLIGFVVL PAFASVHVLC GFIAGLESEF METLSDEEVL LCLTQVLRRV TAPDPVCGGS421HTSHVLLHDA RGSAVGMEGG RPPPQSVGPA GAAAQAQALA GPSLLCSTRV GGRLGPSFLL481TDFSLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
486 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.