Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PANX3 / Pannexin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108062-100
Catalog Number: LS-G108062
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Pannexin 3(PANX3) (NM_052959), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PANX3 | Pannexin 3 | Pannexin-3 | PX3
- Origin Species
- Human
- SwissProt
- Q96QZ0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLAHTAAEY MLSDALLPDR RGPRLKGLRL ELPLDRIVKF VAVGSPLLLM SLAFAQEFSS61GSPISCFSPS NFSIRQAAYV DSSCWDSLLH HKQDGPGQDK MKSLWPHKAL PYSLLALALL121MYLPVLLWQY AAVPALSSDL LFIISELDKS YNRSIRLVQH MLKIRQKSSD PYVFWNELEK181ARKERYFEFP LLERYLACKQ RSHSLVATYL LRNSLLLIFT SATYLYLGHF HLDVFFQEEF241SCSIKTGLLS DETHVPNLIT CRLTSLSIFQ IVSLSSVAIY TILVPVIIYN LTRLCRWDKR301LLSVYEMLPA FDLLSRKMLG CPINDLNVIL LFLRANISEL ISFSWLSVLC VLKDTTTQKH361NIDTVVDFMT LLAGLEPSKP KHLTNSACDE HPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.