Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PANX2 / Pannexin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108151-100
Catalog Number: LS-G108151
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Pannexin 2(PANX2) (NM_052839), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HPANX2 | Pannexin 2 | Pannexin-2 | PANX2 | PX2
- Origin Species
- Human
- SwissProt
- Q96RD6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHHLLEQSAD MATALLAGEK LRELILPGAQ DDKAGALAAL LLQLKLELPF DRVVTIGTVL61VPILLVTLVF TKNFAEEPIY CYTPHNFTRD QALYARGYCW TELRDALPGV DASLWPSLFE121HKFLPYALLA FAAIMYVPAL GWEFLASTRL TSELNFLLQE IDNCYHRAAE GRAPKIEKQI181QSKGPGITER EKREIIENAE KEKSPEQNLF EKYLERRGRS NFLAKLYLAR HVLILLLSAV241PISYLCTYYA TQKQNEFTCA LGASPDGAAG AGPAVRVSCK LPSVQLQRII AGVDIVLLCV301MNLIILVNLI HLFIFRKSNF IFDKLHKVGI KTRRQWRRSQ FCDINILAMF CNENRDHIKS361LNRLDFITNE SDLMYDNVVR QLLAALAQSN HDATPTVRDS GVQTVDPSAN PAEPDGAAEP421PVVKRPRKKM KWIPTSNPLP QPFKEPLAIM RVENSKAEKP KPARRKTATD TLIAPLLDRS481AHHYKGGGGD PGPGPAPAPA PPPAPDKKHA RHFSLDVHPY ILGTKKAKAE AVPAALPASR541SQEGGFLSQA EDCGLGLAPA PIKDAPLPEK EIPYPTEPAR AGLPSGGPFH VRSPPAAPAV601APLTPASLGK AEPLTILSRN ATHPLLHINT LYEAREEEDG GPRLPQDVGD LIAIPAPQQI661LIATFDEPRT VVSTVEFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
677 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.