Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PANK1 / PANK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106436-100
Catalog Number: LS-G106436
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PANK1 (NM_148977), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HPanK1 | HPanK | Pantothenate kinase 1 | Pantothenic acid kinase 1 | PANK | PANK1 | PANK1b | PANK1a | Pantothenate kinase
- Origin Species
- Human
- SwissProt
- Q8TE04
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLKLVGGGGG QDWACSVAGT SLGGEEAAFE VARPGDQGKA GGGSPGWGCA GIPDSAPGAG61VLQAGAVGPA RGGQGAEEVG ESAGGGEERR VRHPQAPALR LLNRKPQGGS GEIKTPENDL121QRGRLSRGPR TAPPAPGMGD RSGQQERSVP HSPGAPVGTS AAAVNGLLHN GFHPPPVQPP181HVCSRGPVGG SDAAPQRLPL LPELQPQPLL PQHDSPAKKC RLRRRMDSGR KNRPPFPWFG241MDIGGTLVKL VYFEPKDITA EEEQEEVENL KSIRKYLTSN TAYGKTGIRD VHLELKNLTM301CGRKGNLHFI RFPSCAMHRF IQMGSEKNFS SLHTTLCATG GGAFKFEEDF RMIADLQLHK361LDELDCLIQG LLYVDSVGFN GKPECYYFEN PTNPELCQKK PYCLDNPYPM LLVNMGSGVS421ILAVYSKDNY KRVTGTSLGG GTFLGLCCLL TGCETFEEAL EMAAKGDSTN VDKLVKDIYG481GDYERFGLQG SAVASSFGNM MSKEKRDSIS KEDLARATLV TITNNIGSIA RMCALNENID541RVVFVGNFLR INMVSMKLLA YAMDFWSKGQ LKALFLEHEG YFGAVGALLE LFKMTDDKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
598 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.