LSBio
PAN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PAN3 antibodies →
$725.00each
Catalog Number: LS-G104973-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAN3 (NM_175854), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HPan3 | TS | PAN3
Origin Species
Human
SwissProt
Q58A45

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDGGALTDTS LTDSYFSTSF IGVNGFGSPV ETKYPLMQRM TNSSSSPSLL NDSAKPYSAH
61DPLTSPASSL FNDFGALNIS QRRKTPNPTA SEFIPKGGST SRLSNVSQSN MSAFSQVFSH
121PSMGSPATAG LAPGMSLSAG SSPLHSPKIT PHTSPAPRRR SHTPNPASYM VPSSASTSVN
181NPVSQTPSSG QVIQKETVGG TTYFYTDTTP APLTGMVFPN YHIYPPTAPH VAYMQPKANA
241PSFFMADELR QELINRHLIT MAQIDQADMP AVPTEVDSYH SLFPLEPLPP PNRIQKSSNF
301GYITSCYKAV NSKDDLPYCL RRIHGFRLVN TKCMVLVDMW KKIQHSNIVT LREVFTTKAF
361AEPSLVFAYD FHAGGETMMS RHFNDPNADA YFTKRKWGQH EGPLPRQHAG LLPESLIWAY
421IVQLSSALRT IHTAGLACRV MDPTKILITG KTRLRVNCVG VFDVLTFDNS QNNNPLALMA
481QYQQADLISL GKVVLALACN SLAGIQRENL QKAMELVTIN YSSDLKNLIL YLLTDQNRMR
541SVNDIMPMIG ARFYTQLDAA QMRNDVIEED LAKEVQNGRL FRLLAKLGTI NERPEFQKDP
601TWSETGDRYL LKLFRDHLFH QVTEAGAPWI DLSHIISCLN KLDAGVPEKI SLISRDEKSV
661LVVTYSDLKR CFENTFQELI AAANGQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

687 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.