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PAMR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAMR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G122778-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAMR1 (NM_001001991), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Inactive serine protease PAMR1 | PAMR1 | DKFZP586H2123 | FP938 | RAMP
Origin Species
Human
NCBI GenBank
AK027841
SwissProt
Q6UXH9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELGCWTQLG LTFLQLLLIS SLPREYTVIN EACPGAEWNI MCRECCEYDQ IECVCPGKRE
61VVGYTIPCCR NEENECDSCL IHPGCTIFEN CKSCRNGSWG GTLDDFYVKG FYCAECRAGW
121YGGDCMRCGQ VLRAPKGQIL LESYPLNAHC EWTIHAKPGF VIQLRFVMLS LEFDYMCQYD
181YVEVRDGDNR DGQIIKRVCG NERPAPIQSI GSSLHVLFHS DGSKNFDGFH AIYEEITACS
241SSPCFHDGTC VLDKAGSYKC ACLAGYTGQR CENLLEERNC SDPGGPVNGY QKITGGPGLI
301NGRHAKIGTV VSFFCNNSYV LSGNEKRTCQ QNGEWSGKQP ICIKACREPK ISDLVRRRVL
361PMQVQSRETP LHQLYSAAFS KQKLQSAPTK KPALPFGDLP MGYQHLHTQL QYECISPFYR
421RLGSSRRTCL RTGKWSGRAP SCIPICGKIE NITAPKTQGL RWPWQAAIYR RTSGVHDGSL
481HKGAWFLVCS GALVNERTVV VAAHCVTDLG KVTMIKTADL KVVLGKFYRD DDRDEKTIQS
541LQISAIILHP NYDPILLDAD IAILKLLDKA RISTRVQPIC LAASRDLSTS FQESHITVAG
601WNVLADVRSP GFKNDTLRSG VVSVVDSLLC EEQHEDHGIP VSVTDNMFCA SWEPTAPSDI
661CTAETGGIAA VSFPGRASPE PRWHLMGLVS WSYDKTCSHR LSTAFTKVLP FKDWIERNMK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

720 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.