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PAM16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAM16 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PAM16 Proteins & Peptides →
$504.00each
Catalog Number: LS-G112881-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAM16 (NM_016069), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CGI-136 | Magmas-like protein | MAGMAS | TIM16 | MAGI-3 | TIMM16 | PAM16
Origin Species
Hu
NCBI GenBank
NM_016069
SwissProt
Q9Y3D7

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
13.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKYLAQIIV MGVQVVGRAF ARALRQEFAA SRAAADARGR AGHRSAAASN LSGLSLQEAQ
61QILNVSKLSP EEVQKNYEHL FKVNDKSVGG SFYLQSKVVR AKERLDEELK IQAQEDREKG
121QMPHT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

125 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.