Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PALS2 / MPP6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101436-100
Catalog Number: LS-G101436
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VAM1(MPP6) (NM_016447), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAGUK protein p55T | MPP6 | VAM1 | p55T | PALS2 | VAM-1 | MAGUK p55 subfamily member 6 | Protein associated with Lin7 2 | Veli-associated MAGUK 1
- Origin Species
- Human
- NCBI GenBank
- AF162130
- SwissProt
- Q9NZW5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQQVLENLTE LPSSTGAEEI DLIFLKGIME NPIVKSLAKA HERLEDSKLE AVSDNNLELV61NEILEDITPL INVDENVAEL VGILKEPHFQ SLLEAHDIVA SKCYDSPPSS PEMNNSSINN121QLLPVDAIRI LGIHKRAGEP LGVTFRVENN DLVIARILHG GMIDRQGLLH VGDIIKEVNG181HEVGNNPKEL QELLKNISGS VTLKILPSYR DTITPQQVFV KCHFDYNPYN DNLIPCKEAG241LKFSKGEILQ IVNREDPNWW QASHVKEGGS AGLIPSQFLE EKRKAFVRRD WDNSGPFCGT301ISSKKKKKMM YLTTRNAEFD RHEIQIYEEV AKMPPFQRKT LVLIGAQGVG RRSLKNRFIV361LNPTRFGTTV PFTSRKPRED EKDGQAYKFV SRSEMEADIK AGKYLEHGEY EGNLYGTKID421SILEVVQTGR TCILDVNPQA LKVLRTSEFM PYVVFIAAPE LETLRAMHKA VVDAGITTKL481LTDSDLKKTV DESARIQRAY NHYFDLIIIN DNLDKAFEKL QTAIEKLRME PQWVPISWVYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
540 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.