Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PALLD / Palladin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128356-100
Catalog Number: LS-G128356
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Palladin(PALLD) (NM_001166109), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Myoneurin | PALLD | Sarcoma antigen NY-SAR-77 | SIH002 | MYN | Palladin | PNCA1 | CGI-151 | CGI151 | KIAA0992
- Origin Species
- Human
- NCBI GenBank
- NM_016081
- SwissProt
- Q8WX93
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPQAQKKTTS VSLTIGSSSP KTGVTTAVIQ PLSVPVQQVH SPTSYLCRPD GTTTAYFPPV61FTKELQNTAV AEGQVVVLEC RVRGAPPLQV QWFRQGSEIQ DSPDFRILQK KPRSTAEPEE121ICTLVIAETF PEDAGIFTCS ARNDYGSATS TAQLVVTSAN TENCSYESMG ESNNDHFQHF181PPPPPILETS SLELASKKPS EIQQVNNPEL GLSRAALQMQ FNAAERETNG VHPSRGVNGL241INGKANSNKS LPTPAVLLSP TKEPPPLLAK PKLGFPKKAS RTARIASDEE IQGTKDAVIQ301DLERKLRFKE DLLNNGQPRL TYEERMARRL LGADSATVFN IQEPEEETAN QEYKVSSCEQ361RLISEIEYRL ERSPVDESGD EVQYGDVPVE NGMAPFFEMK LKHYKIFEGM PVTFTCRVAG421NPKPKIYWFK DGKQISPKSD HYTIQRDLDG TCSLHTTAST LDDDGNYTIM AANPQGRISC481TGRLMVQAVN QRGRSPRSPS GHPHVRRPRS RSRDSGDENE PIQERFFRPH FLQAPGDLTV541QEGKLCRMDC KVSGLPTPDL SWQLDGKPVR PDSAHKMLVR ENGVHSLIIE PVTSRDAGIY601TCIATNRAGQ NSFSLELVVA AKEAHKPPVF IEKLQNTGVA DGYPVRLECR VLGVPPPQIF661WKKENESLTH STDRVSMHQD NHGYICLLIQ GATKEDAGWY TVSAKNEAGI VSCTARLDVY721TQWHQQSQST KPKKVRPSAS RYAALSDQGL DIKAAFQPEA NPSHLTLNTA LVESEDLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
777 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.