Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PALLD / Palladin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128305-100
Catalog Number: LS-G128305
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Palladin(PALLD) (NM_001166110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Myoneurin | PALLD | Sarcoma antigen NY-SAR-77 | SIH002 | MYN | Palladin | PNCA1 | CGI-151 | CGI151 | KIAA0992
- Origin Species
- Human
- NCBI GenBank
- NM_016081
- SwissProt
- Q8WX93
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSALASRSAP AMQSSGSFNY ARPKQFIAAQ NLGPASGHGT PASSPSSSSL PSPMSPTPRQ61FGRAPVPPFA QPFGAEPEAP WGSSSPSPPP PPPPVFSPTA AFPVPDVFPL PPPPPPLPSP121GQASHCSSPA TRFGHSQTPA AFLSALLPSQ PPPAAVNALG LPKGVTPAGF PKKASRTARI181ASDEEIQGTK DAVIQDLERK LRFKEDLLNN GQPRLTYEER MARRLLGADS ATVFNIQEPE241EETANQEYKV SSCEQRLISE IEYRLERSPV DESGDEVQYG DVPVENGMAP FFEMKLKHYK301IFEGMPVTFT CRVAGNPKPK IYWFKDGKQI SPKSDHYTIQ RDLDGTCSLH TTASTLDDDG361NYTIMAANPQ GRISCTGRLM VQAVNQRGRS PRSPSGHPHV RRPRSRSRDS GDENEPIQER421FFRPHFLQAP GDLTVQEGKL CRMDCKVSGL PTPDLSWQLD GKPVRPDSAH KMLVRENGVH481SLIIEPVTSR DAGIYTCIAT NRAGQNSFSL ELVVAAKEAH KPPVFIEKLQ NTGVADGYPV541RLECRVLGVP PPQIFWKKEN ESLTHSTDRV SMHQDNHGYI CLLIQGATKE DAGWYTVSAK601NEAGIVSCTA RLDVYTQWHQ QSQSTKPKKV RPSASRYAAL SDQGLDIKAA FQPEANPSHL661TLNTALVESE DLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
672 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.