Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PALD1 / Paladin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113946-100
Catalog Number: LS-G113946
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PALD(PALD1) (NM_014431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1274 | PALD1 | Palladin | Paladin | PALD
- Origin Species
- Human
- NCBI GenBank
- AB033100
- SwissProt
- Q9ULE6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTTASTAQQ TVSAGTPFEG LQGSGTMDSR HSVSIHSFQS TSLHNSKAKS IIPNKVAPVV61ITYNCKEEFQ IHDELLKAHY TLGRLSDNTP EHYLVQGRYF LVRDVTEKMD VLGTVGSCGA121PNFRQVQGGL TVFGMGQPSL SGFRRVLQKL QKDGHRECVI FCVREEPVLF LRADEDFVSY181TPRDKQNLHE NLQGLGPGVR VESLELAIRK EIHDFAQLSE NTYHVYHNTE DLWGEPHAVA241IHGEDDLHVT EEVYKRPLFL QPTYRYHRLP LPEQGSPLEA QLDAFVSVLR ETPSLLQLRD301AHGPPPALVF SCQMGVGRTN LGMVLGTLIL LHRSGTTSQP EAAPTQAKPL PMEQFQVIQS361FLRMVPQGRR MVEEVDRAIT ACAELHDLKE VVLENQKKLE GIRPESPAQG SGSRHSVWQR421ALWSLERYFY LILFNYYLHE QYPLAFALSF SRWLCAHPEL YRLPVTLSSA GPVAPRDLIA481RGSLREDDLV SPDALSTVRE MDVANFRRVP RMPIYGTAQP SAKALGSILA YLTDAKRRLR541KVVWVSLREE AVLECDGHTY SLRWPGPPVA PDQLETLEAQ LKAHLSEPPP GKEGPLTYRF601QTCLTMQEVF SQHRRACPGL TYHRIPMPDF CAPREEDFDQ LLEALRAALS KDPGTGFVFS661CLSGQGRTTT AMVVAVLAFW HIQGFPEVGE EELVSVPDAK FTKGEFQVVM KVVQLLPDGH721RVKKEVDAAL DTVSETMTPM HYHLREIIIC TYRQAKAAKE AQEMRRLQLR SLQYLERYVC781LILFNAYLHL EKADSWQRPF STWMQEVASK AGIYEILNEL GFPELESGED QPFSRLRYRW841QEQSCSLEPS APEDLLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
856 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.