Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PAL / PAM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107177-100
Catalog Number: LS-G107177
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAM (NM_138821), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PAL | PAM | Peptidylglycine 2-hydroxylase | Peptidylamidoglycolate lyase | PHM
- Origin Species
- Human
- NCBI GenBank
- NM_000919
- SwissProt
- P19021
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 94.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV PIDSSDFALD61IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT VHHMLLFGCN MPSSTGSYWF121CDEGTCTDKA NILYAWARNA PPTRLPKGVG FRVGGETGSK YFVLQVHYGD ISAFRDNNKD181CSGVSLHLTR LPQPLIAGMY LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH241LGKVVSGYRV RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH301IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP VKSDMVMMHE361HHKETEYKDK IPLLQQPKRE EEEVLDQDFH MEEALDWPGV YLLPGQVSGV ALDPKNNLVI421FHRGDHVWDG NSFDSKFVYQ QIGLGPIEED TILVIDPNNA AVLQSSGKNL FYLPHGLSID481KDGNYWVTDV ALHQVFKLDP NNKEGPVLIL GRSMQPGSDQ NHFCQPTDVA VDPGTGAIYV541SDGYCNSRIV QFSPSGKFIT QWGEESSGSS PLPGQFTVPH SLALVPLLGQ LCVADRENGR601IQCFKTDTKE FVREIKHSSF GRNVFAISYI PGLLFAVNGK PHFGDQEPVQ GFVMNFSNGE661IIDIFKPVRK HFDMPHDIVA SEDGTVYIGD AHTNTVWKFT LTEKLEHRSV KKAGIEVQEI721KEAEAVVETK MENKPTSSEL QKMQEKQKLI KEPGSGVPVV LITTLLVIPV VVLLAIAIFI781RWKKSRAFGD SEHKLETSSG RVLGRFRGKG SGGLNLGNFF ASRKGYSRKG FDRLSTEGSD841QEKEDDGSES EEEYSAPLPA LAPSSSTRTR PLEQKLISEE DLAANDILDY KDDDDKV
897 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.