Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PAK1IP1 / HPIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112127-100
Catalog Number: LS-G112127
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAK1 interacting protein 1(PAK1IP1) (NM_017906), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA421M1.5 | HPIP1 | MAK11 | PAK/PLC-interacting protein 1 | PAK1-interacting protein 1 | PAK1IP1 | RP11-421M1.5 | PIP1 | PAK1 interacting protein 1 | WDR84
- Origin Species
- Human
- NCBI GenBank
- NM_017906
- SwissProt
- Q9NWT1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELVAGCYEQ VLFGFAVHPE PEACGDHEQW TLVADFTHHA HTASLSAVAV NSRFVVTGSK61DETIHIYDMK KKIEHGALVH HSGTITCLKF YGNRHLISGA EDGLICIWDA KKWECLKSIK121AHKGQVTFLS IHPSGKLALS VGTDKTLRTW NLVEGRSAFI KNIKQNAHIV EWSPRGEQYV181VIIQNKIDIY QLDTASISGT VTNEKRISSV KFLSESVLAV AGDEEVIRFF DCDSLVCLCE241FKAHENRVKD MFSFEIPEHH VIVSASSDGF IKMWKLEQDK KVPPSLLCEI NTNARLTCLG301VWLDKVADMK ESLPPAAEPS PVSKEQSKIG KKEPGDTVHK EEKRSKPNTK KRGLTGDSKK361ATKESGLIST KKRKMVEMLE KKRKKKKIKT MQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.