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PAIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104122-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAIP1 (NM_182789), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PABC1-interacting protein 1 | PABP-interacting protein 1 | PAIP1 | PAIP-1
Origin Species
Human
NCBI GenBank
NM_006451
SwissProt
Q9H074

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDGFDRAPE QTRPLRAPPS SQDKIPQQNS ESAMAKPQVV VAPVLMSKLS VNAPEFYPSG
61YSSSYTESYE DGCEDYPTLS EYVQDFLNHL TEQPGSFETE IEQFAETLNG CVTTDDALQE
121LVELIYQQAT SIPNFSYMGA RLCNYLSHHL TISPQSGNFR QLLLQRCRTE YEVKDQAAKG
181DEVTRKRFHA FVLFLGELYL NLEIKGTNGQ VTRADILQVG LRELLNALFS NPMDDNLICA
241VKLLKLTGSV LEDAWKEKGK MDMEEIIQRI ENVVLDANCS RDVKQMLLKL VELRSSNWGR
301VHATSTYREA TPENDPNYFM NEPTFYTSDG VPFTAADPDY QEKYQELLER EDFFPDYEEN
361GTDLSGAGDP YLDDIDDEMD PEIEEAYEKF CLESERKRKQ TRTRPLEQKL ISEEDLAAND
421ILDYKDDDDK V

431 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.