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PADI6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PADI6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102813-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PADI6 (NM_207421), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HPADVI | Peptidylarginine deiminase VI | PADI6
Origin Species
Human
NCBI GenBank
NM_207421
SwissProt
Q6TGC4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVSVEGRAMS FQSIIHLSLD SPVHAVCVLG TEICLDLSGC APQKCQCFTI HGSGRVLIDV
61ANTVISEKED ATIWWPLSDP TYATVKMTSP SPSVDADKVS VTYYGPNEDA PVGTAVLYLT
121GIEVSLEVDI YRNGQVEMSS DKQAKKKWIW GPSGWGAILL VNCNPADVGQ QLEDKKTKKV
181IFSEEITNLS QMTLNVQGPS CILKKYRLVL HTSKEESKKA RVYWPQKDNS STFELVLGPD
241QHAYTLALLG NHLKETFYVE AIAFPSAEFS GLISYSVSLV EESQDPSIPE TVLYKDTVVF
301RVAPCVFIPC TQVPLEVYLC RELQLQGFVD TVTKLSEKSN SQVASVYEDP NRLGRWLQDE
361MAFCYTQAPH KTTSLILDTP QAADLDEFPM KYSLSPGIGY MIQDTEDHKV ASMDSIGNLM
421VSPPVKVQGK EYPLGRVLIG SSFYPSAEGR AMSKTLRDFL YAQQVQAPVE LYSDWLMTGH
481VDEFMCFIPT DDKNEGKKGF LLLLASPSAC YKLFREKQKE GYGDALLFDE LRADQLLSNG
541REAKTIDQLL ADESLKKQNE YVEKCIHLNR DILKTELGLV EQDIIEIPQL FCLEKLTNIP
601SDQQPKRSFA RPYFPDLLRM IVMGKNLGIP KPFGPQIKGT CCLEEKICCL LEPLGFKCTF
661INDFDCYLTE VGDICACANI RRVPFAFKWW KMVP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

694 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.