Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PAD2 / PADI2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114677-100
Catalog Number: LS-G114677
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PADI2(PADI2) (NM_007365), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PAD-H19 | PDI2 | PAD2 | Protein arginine deiminase | KIAA0994 | PADI2 | Peptidylarginine deiminase II
- Origin Species
- Human
- NCBI GenBank
- AB023211
- SwissProt
- Q9Y2J8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLRERTVRLQ YGSRVEAVYV LGTYLWTDVY SAAPAGAQTF SLKHSEHVWV EVVRDGEAEE61VATNGKQRWL LSPSTTLRVT MSQASTEASS DKVTVNYYDE EGSIPIDQAG LFLTAIEISL121DVDADRDGVV EKNNPKKASW TWGPEGQGAI LLVNCDRETP WLPKEDCRDE KVYSKEDLKD181MSQMILRTKG PDRLPAGYEI VLYISMSDSD KVGVFYVENP FFGQRYIHIL GRRKLYHVVK241YTGGSAELLF FVEGLCFPDE GFSGLVSIHV SLLEYMAQDI PLTPIFTDTV IFRIAPWIMT301PNILPPVSVF VCCMKDNYLF LKEVKNLVEK TNCELKVCFQ YLNRGDRWIQ DEIEFGYIEA361PHKGFPVVLD SPRDGNLKDF PVKELLGPDF GYVTREPLFE SVTSLDSFGN LEVSPPVTVN421GKTYPLGRIL IGSSFPLSGG RRMTKVVRDF LKAQQVQAPV ELYSDWLTVG HVDEFMSFVP481IPGTKKFLLL MASTSACYKL FREKQKDGHG EAIMFKGLGG MSSKRITINK ILSNESLVQE541NLYFQRCLDW NRDILKKELG LTEQDIIDLP ALFKMDEDHR ARAFFPNMVN MIVLDKDLGI601PKPFGPQVEE ECCLEMHVRG LLEPLGLECT FIDDISAYHK FLGEVHCGTN VRRKPFTFKW661WHMVPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
665 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.