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PACS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PACS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113388-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PACS2 (NM_015197), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0602 | PACS1-like protein | PACS2 | PACS-2 | PACS1L
Origin Species
Human
NCBI GenBank
AB011174
SwissProt
Q86VP3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAERGRLGLP GAPGALNTPV PMNLFATWEV DGSSPSCVPR LCSLTLKKLV VFKELEKELI
61SVVIAVKMQG SKRILRSHEI VLPPSGQVET DLALTFSLQY PHFLKREGNK LQIMLQRRKR
121YKNRTILGYK TLAAGSISMA EVMQHPSEGG QVLSLCSSIK EAPVKAAEIW IASLSSQPID
181HEDSTMQAGP KAKSTDNYSE EEYESFSSEQ EASDDAVQGQ DLDEDDFDVG KPKKQRRSIV
241RTTSMTRQQN FKQKVVALLR RFKVSDEVLD SEQDPAEHIP EAEEDLDLLY DTLDMEHPSD
301SGPDMEDDDS VLSTPKPKLR PYFEGLSHSS SQTEIGSIHS ARSHKEPPSP ADVPEKTRSL
361GGRQPSDSVS DTVALGVPGP REHPGQPEDS PEAEASTLDV FTERLPPSGR ITKTESLVIP
421STRSEGKQAG RRGRSTSLKE RQAARPQNER ANSLDNERCP DARSQLQIPR KTVYDQLNHI
481LISDDQLPEN IILVNTSDWQ GQFLSDVLQR HTLPVVCTCS PADVQAAFST IVSRIQRYCN
541CNSQPPTPVK IAVAGAQHYL SAILRLFVEQ LSHKTPDWLG YMRFLVIPLG SHPVARYLGS
601VDYRYNNFFQ DLAWRDLFNK LEAQSAVQDT PDIVSRITQY IAGANCAHQL PIAEAMLTYK
661QKSPDEESSQ KFIPFVGVVK VGIVEPSSAT SGDSDDAAPS GSGTLSSTPP SASPAAKEAS
721PTPPSSPSVS GGLSSPSQGV GAELMGLQVD YWTAAQPADR KRDAEKKDLP VTKNTLKCTF
781RSLQVSRLPS SGEAAATPTM SMTVVTKEKN KKVMFLPKKA KDKDVESKSQ CIEGISRLIC
841TARQQQNMLR VLIDGVECSD VKFFQLAAQW SSHVKHFPIC IFGHSKATF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

889 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.