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PABPC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PABPC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107740-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PABPC5 (NM_080832), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PABP-5 | PABP5 | PABPC5 | Poly(A)-binding protein 5
Origin Species
Human
SwissProt
Q96DU9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MGSGEPNPAG KKKKYLKAAL YVGDLDPDVT EDMLYKKFRP AGPLRFTRIC RDPVTRSPLG
61YGYVNFRFPA DAEWALNTMN FDLINGKPFR LMWSQPDDRL RKSGVGNIFI KNLDKSIDNR
121ALFYLFSAFG NILSCKVVCD DNGSKGYAYV HFDSLAAANR AIWHMNGVRL NNRQVYVGRF
181KFPEERAAEV RTRDRATFTN VFVKNIGDDI DDEKLKELFC EYGPTESVKV IRDASGKSKG
241FGFVRYETHE AAQKAVLDLH GKSIDGKVLY VGRAQKKIER LAELRRRFER LRLKEKSRPP
301GVPIYIKNLD ETINDEKLKE EFSSFGSISR AKVMMEVGQG KGFGVVCFSS FEEATKAVDE
361MNGRIVGSKP LHVTLGQARR RC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

382 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.