Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
P2RY6 / P2Y6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104866-100
Catalog Number: LS-G104866
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human P2Y6(P2RY6) (NM_176796), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-coupled nucleotide receptor | p2 purinoceptor | p2y3 receptor | p2Y purinoceptor 6 | Purinergic receptor p2y6 | p2RY6 | p2y3 and chick | p2Y6 receptor | p2Y6 | Purinoceptor p2y6
- Origin Species
- Human
- NCBI GenBank
- NM_004154
- SwissProt
- Q15077
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEWDNGTGQA LGLPPTTCVY RENFKQLLLP PVYSAVLAAG LPLNICVITQ ICTSRRALTR61TAVYTLNLAL ADLLYACSLP LLIYNYAQGD HWPFGDFACR LVRFLFYANL HGSILFLTCI121SFQRYLGICH PLAPWHKRGG RRAAWLVCVA VWLAVTTQCL PTAIFAATGI QRNRTVCYDL181SPPALATHYM PYGMALTVIG FLLPFAALLA CYCLLACRLC RQDGPAEPVA QERRGKAARM241AVVVAAAFAI SFLPFHITKT AYLAVRSTPG VPCTVLEAFA AAYKGTRPFA SANSVLDPIL301FYFTQKKFRR RPHELLQKLT AKWQRQGRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
328 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.