Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
P2RY2 / P2Y2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104957-100
Catalog Number: LS-G104957
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human P2Y2(P2RY2) (NM_176071), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATP receptor | p2U | p2U nucleotide receptor | p2UR | p2Y2 | p2RU1 | Purinergic receptor | p2RY2 | p2u purinergic receptor 1 | p2U1 | p2Y purinoceptor 2 | Purinergic receptor P2Y2 | Purinoceptor P2Y2 | HP2U | p2U purinoceptor 1 | p2Y2R
- Origin Species
- Human
- NCBI GenBank
- NM_002564
- SwissProt
- P41231
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAADLGPWND TINGTWDGDE LGYRCRFNED FKYVLLPVSY GVVCVPGLCL NAVALYIFLC61RLKTWNASTT YMFHLAVSDA LYAASLPLLV YYYARGDHWP FSTVLCKLVR FLFYTNLYCS121ILFLTCISVH RCLGVLRPLR SLRWGRARYA RRVAGAVWVL VLACQAPVLY FVTTSARGGR181VTCHDTSAPE LFSRFVAYSS VMLGLLFAVP FAVILVCYVL MARRLLKPAY GTSGGLPRAK241RKSVRTIAVV LAVFALCFLP FHVTRTLYYS FRSLDLSCHT LNAINMAYKV TRPLASANSC301LDPVLYFLAG QRLVRFARDA KPPTGPSPAT PARRRLGLRR SDRTDMQRIE DVLGSSEDSR361RTESTPAGSE NTKDIRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
377 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.