Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
P2RY14 / GPR105 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113633-100
Catalog Number: LS-G113633
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR105(P2RY14) (NM_014879), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 105 | p2Y purinoceptor 14 | p2Y(14) receptor | p2Y14 receptor | UDP-glucose receptor | p2Y14 | VTR 15-20 receptor | G protein-coupled receptor 105 | GPR105 | KIAA0001 | p2RY14
- Origin Species
- Human
- NCBI GenBank
- NM_014879
- SwissProt
- Q15391
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MINSTSTQPP DESCSQNLLI TQQIIPVLYC MVFIAGILLN GVSGWIFFYV PSSESFIIYL61KNIVIADFVM SLTFPFKILG DSGLGPWQLN VFVCRVSAVL FYVNMYVSIV FFGLISFDRY121YKIVKPLWTS FIQSVSYSKL LSVIVWMLML LLAVPNIILT NQSVREVTQI KCIELKSELG181RKWHKASNYI FVAIFWIVFL LLIVFYTAIT KKIFKSHLKS SRNSTSVKKK SSRNIFSIVF241VFFVCFVPYH IARIPYTKSQ TEAHYSCQSK EILRYMKEFT LLLSAANVCL DPIIYFFLCQ301PFREILCKKL HIPLKAQNDL DISRIKRGNT TLESTDTLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
338 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.