Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
P2RY12 / P2Y12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101833-100
Catalog Number: LS-G101833
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human P2Y12(P2RY12) (NM_022788), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADP-glucose receptor | ADPG-R | p2Y(12)R | p2Y(ADP) | p2T(AC) | p2Y12 | Platelet ADP receptor | SP1999 | p2RY12 | p2Y purinoceptor 12 | Purinergic receptor P2RY12 | BDPLT8 | Gi-coupled ADP receptor HORK3 | HORK3 | p2Y(AC) | p2Y(cyc) | p2Y12 platelet ADP receptor
- Origin Species
- Human
- NCBI GenBank
- NM_022788
- SwissProt
- Q9H244
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQAVDNLTSA PGNTSLCTRD YKITQVLFPL LYTVLFFVGL ITNGLAMRIF FQIRSKSNFI61IFLKNTVISD LLMILTFPFK ILSDAKLGTG PLRTFVCQVT SVIFYFTMYI SISFLGLITI121DRYQKTTRPF KTSNPKNLLG AKILSVVIWA FMFLLSLPNM ILTNRQPRDK NVKKCSFLKS181EFGLVWHEIV NYICQVIFWI NFLIVIVCYT LITKELYRSY VRTRGVGKVP RKKVNVKVFI241IIAVFFICFV PFHFARIPYT LSQTRDVFDC TAENTLFYVK ESTLWLTSLN ACLDPFIYFF301LCKSFRNSLI SMLKCPNSAT SLSQDNRKKE QDGGDPNEET PMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
342 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.