Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
P2RX5 / P2X5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99789-100
Catalog Number: LS-G99789
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human P2X5(P2RX5) (NM_002561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATP receptor | ATP receptor subunit | LRH-1 | p2X5R | p2X purinoceptor 5 | p2X5 purinoceptor | Purinergic receptor | Ionotropic ATP receptor P2X5 | p2RX5 | p2X receptor 5 | p2X5
- NCBI GenBank
- NM_002561
- SwissProt
- Q93086
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGQAGCKGLC LSLFDYKTEK YVIAKNKKVG LLYRLLQASI LAYLVVWVFL IKKGYQDVDT61SLQSAVITKV KGVAFTNTSD LGQRIWDVAD YVIPAQGENV FFVVTNLIVT PNQRQNVCAE121NEGIPDGACS KDSDCHAGEA VTAGNGVKTG RCLRRENLAR GTCEIFAWCP LETSSRPEEP181FLKEAEDFTI FIKNHIRFPK FNFSKSNVMD VKDRSFLKSC HFGPKNHYCP IFRLGSVIRW241AGSDFQDIAL EGGVIGINIE WNCDLDKAAS ECHPHYSFSR LDNKLSKSVS SGYNFRFARY301YRDAAGVEFR TLMKAYGIRF DVMVNGKGAF FCDLVLIYLI KKREFYRDKK YEEVRGLEDS361SQEAEDEASG LGLSEQLTSG PGLLGMPEQQ ELQEPPEAKR GSSSQKGNGS VCPQLLEPHR421STTRTRPLEQKLISEEDLAANDILDYKDDDDKV
422 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.