Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
P2RX2 / P2X2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112724-100
Catalog Number: LS-G112724
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human P2X2(P2RX2) (NM_016318), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATP receptor | p2X2 | p2X Receptor | subunit 2 | Purinergic receptor | p2RX2 | p2X receptor 2 | p2X purinoceptor 2 | p2X2 purinoceptor
- Origin Species
- Human
- NCBI GenBank
- NM_012226
- SwissProt
- Q9UBL9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAQPKYPA GATARRLARG CWSALWDYET PKVIVVRNRR LGVLYRAVQL LILLYFVWYV61FIVQKSYQES ETGPESSIIT KVKGITTSEH KVWDVEEYVK PPESIRVHNA TCLSDADCVA121GELDMLGNGL RTGRCVPYYQ GPSKTCEVFG WCPVEDGASV SQFLGTMAPN FTILIKNSIH181YPKFHFSKGN IADRTDGYLK RCTFHEASDL YCPIFKLGFI VEKAGESFTE LAHKGGVIGV241IINWDCDLDL PASECNPKYS FRRLDPKHVP ASSGYNFRFA KYYKINGTTT RTLIKAYGIR301IDVIVHGQAG KFSLIPTIIN LATALTSVGV GSFLCDWILL TFMNKNKVYS HKKFDKVCTP361SHPSGSWPVT LARVLGQAPP EPGHRSEDQH PSPPSGQEGQ QGAECGPAFP PLRPCPISAP421SEQMVDTPAS EPAQASTPTD PKGLAQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
447 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.