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OXCT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OXCT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OXCT2 antibodies →
$504.00each
Catalog Number: LS-G101791-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OXCT2 (NM_022120), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
3-oxoacid CoA-transferase 2A | 3-oxoacid CoA transferase 2 | 3-oxoacid-CoA transferase 2A | FKSG25 | SCOT-t | OXCT2 | SCOTT
Origin Species
Human
NCBI GenBank
NM_022120
SwissProt
Q9BYC2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAALRLLASV LGRGVPAGGS GLALSQGCAR CFATSPRLRA KFYADPVEMV KDISDGATVM
61IGGFGLCGIP ENLIAALLRT RVKDLQVVSS NVGVEDFGLG LLLAARQVRR IVCSYVGENT
121LCESQYLAGE LELELTPQGT LAERIRAGGA GVPAFYTPTG YGTLVQEGGA PIRYTPDGHL
181ALMSQPREVR EFNGDHFLLE RAIRADFALV KGWKADRAGN VVFRRSARNF NVPMCKAADV
241TAVEVEEIVE VGAFPPEDIH VPNIYVDRVI KGQKYEKRIE RLTILKEEDG DAGKEEDART
301RIIRRAALEF EDGMYANLGI GIPLLASNFI SPSMTVHLHS ENGILGLGPF PTEDEVDADL
361INAGKQTVTV LPGGCFFASD DSFAMIRGGH IQLTMLGAMQ VSKYGDLANW MIPGKKVKGM
421GGAMDLVSSQ KTRVVVTMQH CTKDNTPKIM EKCTMPLTGK RCVDRIITEK AVFDVHRKKE
481LTLRELWEGL TVDDIKKSTG CAFAVSPNLR PMQQVAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

517 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.