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OVCH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OVCH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104023-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OVCH1 (NM_183378), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ovochymase-1 | OVCH | OVCH1 | Ovochymase 1
Origin Species
Human
NCBI GenBank
NM_183378
SwissProt
Q7RTY7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGLLASAGLL LLLVIGHPRS LGLKCGIRMV NMKSKEPAVG SRFFSRISSW RNSTVTGHPW
61QVSLKSDEHH FCGGSLIQED RVVTAAHCLD SLSEKQLKNI TVTSGEYSLF QKDKQEQNIP
121VSKIITHPEY NSREYMSPDI ALLYLKHKVK FGNAVQPICL PDSDDKVEPG ILCLSSGWGK
181ISKTSEYSNV LQEMELPIMD DRACNTVLKS MNLPPLGRTM LCAGFPDWGM DACQGDSGGP
241LVCRRGGGIW ILAGITSWVA GCAGGSVPVR NNHVKASLGI FSKVSELMDF ITQNLFTGLD
301RGQPLSKVGS RYITKALSSV QEVNGSQRGK GILDMEKQVG CDHDYVSLRS SSGVLFNQRS
361LMEDDGKQNK RVCGKILPSP LLAETSEAMV PFVSDTEDSG SGFELTVTAV QKSEAGSGCG
421SLAILVEEGT NHSAKYPDLY PSNTRCHWFI CAPEKHIIKL TFEDFAVKFS PNCIYDAVVI
481YGDSEEKHKL AKLCGMLTIT SIFSSSNMTV IYFKSDGKNR LQGFKARFTI LPSESLNKFE
541PKLPPQNNPV STVKAILHDV CGIPPFSPQW LSRRIAGGEE ACPHCWPWQV GLRFLGDYQC
601GGAIINPVWI LTAAHCVQLK NNPLSWTIIA GDHDRNLKES TEQVRRAKHI IVHEDFNTLS
661YDSDIALIQL SSPLEYNSVV RPVCLPHSAE PLFSSEICAV TGWGSISADG GLASRLQQIQ
721VHVLEREVCE HTYYSAHPGG ITEKMICAGF AASGEKDFCQ GDSGGPLVCR HENGPFVLYG
781IVSWGAGCVQ PWKPGVFARV MIFLDWIQSK INGPASLQTN NKCKTLKQQL PPPTPSPDSA
841SWPGCCSEAE LEKPRGFFPT PRYLLDYRGR LECSWVLRVS ASSMAKFTIE YLSLLGSPVC
901QDSVLIIYEE RHSKRKTAGG LHGRRLYSMT FMSPGPLVRV TFHALVRGAF GISYIVLKVL
961GPKDSKITRL SQSSNREHLV PCEDVLLTKP EGIMQIPRNS HRTTMGCQWR LVAPLNHIIQ
1021LNIINFPMKP TTFVCHGHLR VYEGFGPGKK LIASFAGTLA MILTKDILKR EKLNFINTYI
1081MHIWENSVYD NVRSVGKRKQ KKFASNLSYS MEAEKSRIQV PADLVPAKGS LSGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1134 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.