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OTOP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OTOP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104749-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OTOP2 (NM_178160), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Otopetrin 2 | Otopetrin-2 | OTOP2
Origin Species
Human
SwissProt
Q7RTS6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEELAQGPK ESPPAPRAGP REVWKKGGRL LSVLLAVNVL LLACTLISGG AFNKVAVYDT
61DVFALLTAMM LLATLWILFY LLRTVRCPCA VPYRDAHAGP IWLRGGLVLF GICTLIMDVF
121KTGYYSSFFE CQSAIKILHP LIQAVFVIIQ TYFLWVSAKD CVHVHLDLTW CGLMFTLTTN
181LAIWMAAVVD ESVHQSHSYS SSHSNASHAR LISDQHADNP VGGDSCLCST AVCQIFQQGY
241FYLYPFNIEY SLFASTMLYV MWKNVGRFLA STPGHSHTPT PVSLFRETFF AGPVLGLLLF
301VVGLAVFIIY EVQVSGDGSR TRQALVIYYS FNIVCLGLTT LVSLSGSIIY RFDRRAMDHH
361KNPTRTLDVA LLMGAALGQY AISYYSIVAV VAGTPQDLLA GLNLTHALLM IAQHTFQNMF
421IIESLHRGPP GAEPHSTHPK EPCQDLTFTN LDALHTLSAC PPNPGLVSPS PSDQREAVAI
481VSTPRSQWRR QCLKDISLFL LLCNVILWIM PAFGARPHFS NTVEVDFYGY SLWAVIVNIC
541LPFGIFYRMH AVSSLLEVYV LS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

562 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.