Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Osteoglycin / Mimecan Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102100-100
Catalog Number: LS-G102100
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OIF(OGN) (NM_033014), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OIF | Osteoglycin | SLRR3A | Mimecan proteoglycan | OG | OGN | Mimecan | Osteoinductive factor
- Origin Species
- Human
- NCBI GenBank
- NM_024416
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 31.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKTLQSTLLL LLLVPLIKPA PPTQQDSRII YDYGTDNFEE SIFSQDYEDK YLDGKNIKEK61ETVIIPNEKS LQLQKDEAIT PLPPKKENDE MPTCLLCVCL SGSVYCEEVD IDAVPPLPKE121SAYLYARFNK IKKLTAKDFA DIPNLRRLDF TGNLIEDIED GTFSKLSLLE ELSLAENQLL181KLPVLPPKLT LFNAKYNKIK SRGIKANAFK KLNNLTFLYL DHNALESVPL NLPESLRVIH241LQFNNIASIT DDTFCKANDT SYIRDRIEEI RLEGNPIVLG KHPNSFICLK RLPIGSYFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
298 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.