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OSGIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OSGIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114295-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OSGIN1 (NM_013370), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BDGI | HuOKL38 | OSGIN1 | OKL38
Origin Species
Human
NCBI GenBank
NM_013370
SwissProt
Q9UJX0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKWRPRGCC RGNMQCRQEV PATLTSSELF STRNQPQPQP QPLLADAPVP WAVASRMCLT
61PGQGCGHQGQ DEGPLPAPSP PPAMSSSRKD HLGASSSEPL PVIIVGNGPS GICLSYLLSG
121YTPYTKPDAI HPHPLLQRKL TEAPGVSILD QDLDYLSEGL EGRSQSPVAL LFDALLRPDT
181DFGGNMKSVL TWKHRKEHAI PHVVLGRNLP GGAWHSIEGS MVILSQGQWM GLPDLEVKDW
241MQKKRRGLRN SRATAGDIAH YYRDYVVKKG LGHNFVSGAV VTAVEWGTPD PSSCGAQDSS
301PLFQVSGFLT RNQAQQPFSL WARNVVLATG TFDSPARLGI PGEALPFIHH ELSALEAATR
361VGAVTPASDP VLIIGAGLSA ADAVLYARHY NIPVIHAFRR AVDDPGLVFN QLPKMLYPEY
421HKVHQMMREQ SILSPSPYEG YRSLPRHQLL CFKEDCQAVF QDLEGVEKVF GVSLVLVLIG
481SHPDLSFLPG AGADFAVDPD QPLSAKRNPI DVDPFTYQST RQEGLYAMGP LAGDNFVRFV
541QGGALAVASS LLRKETRKPP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

560 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.