Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
OSBPL1A / ORP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107849-100
Catalog Number: LS-G107849
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ORP1(OSBPL1A) (NM_080597), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ORP-1 | ORP1 | OSBP-related protein 1 | OSBP8 | OSBPL1A | OSBPL1 | OSBPL1B
- Origin Species
- Human
- NCBI GenBank
- NM_018030
- SwissProt
- Q9BXW6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNTEAEQQLL HHARNGNAEE VRQLLETMAR NEVIADINCK GRSKSNLGWT PLHLACYFGH61RQVVQDLLKA GAEVNVLNDM GDTPLHRAAF TGRKELVMLL LEYNADTTIV NGSGQTAKEV121THAEEIRSML EAVERTQQRK LEELLLAAAR EGKTTELTAL LNRPNPPDVN CSDQLGNTPL181HCAAYRAHKQ CALKLLRSGA DPNLKNKNDQ KPLDLAQGAE MKHILVGNKV IYKALKRYEG241PLWKSSRFFG WRLFWVVLEH GVLSWYRKQP DAVHNIYRQG CKHLTQAVCT VKSTDSCLFF301IKCFDDTIHG FRVPKNSLQQ SREDWLEAIE EHSAYSTHYC SQDQLTDEEE EDTVSAADLK361KSLEKAQSCQ QRLDREISNF LKMIKECDMA KEMLPSFLQK VEVVSEASRE TCVALTDCLN421LFTKQEGVRN FKLEQEQEKN KILSEALETL ATEHHELEQS LVKGSPPASI LSEDEFYDAL481SDSESERSLS RLEAVTARSF EEEGEHLGSR KHRMSEEKDC GGGDALSNGI KKHRTSLPSP541MFSRNDFSIW SILRKCIGME LSKITMPVIF NEPLSFLQRL TEYMEHTYLI HKASSLSDPV601ERMQCVAAFA VSAVASQWER TGKPFNPLLG ETYELVRDDL GFRLISEQVS HHPPISAFHA661EGLNNDFIFH GSIYPKLKFW GKSVEAEPKG TITLELLEHN EAYTWTNPTC CVHNIIVGKL721WIEQYGNVEI INHKTGDKCV LNFKPCGLFG KELHKVEGYI QDKSKKKLCA LYGKWTECLY781SVDPATFDAY KKNDKKNTEE KKNSKQMSTS EELDEMPVPD SESVFIIPGS VLLWRIAPRP841PNSAQMYNFT SFAMVLNEVD KDMESVIPKT DCRLRPDIRA MENGEIDQAS EEKKRLEEKQ901RAARKNRSKS EEDWKTRWFH QGPNPYNGAQ DWIYSGSYWD RNYFNLPDIYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
950 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.