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OSBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OSBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109422-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OSBP2 (NM_030758), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1664 | ORP4 | OSBP-related protein 4 | OSBP2 | ORP-4 | Oxysterol binding protein 2 | OSBPL4 | OSBPL1 | Oxysterol-binding protein 2
Origin Species
Human
NCBI GenBank
NM_030758
SwissProt
Q969R2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKAAAPSRG GGCGGRSRGL SSLFTVVPCL SCHTAAPGMS ASTSGSGPEP KPQPQPVPEP
61ERGPLSEQVS EAVSEAVPRS EPVSETTSEP EPGAGQPSEL LQGSRPGSES SSGVGAGPFT
121KAASEPLSRA VGSATFLRPE SGSLPALKPL PLLRPGQAKT PLGVPMSGTG TTSSAPLALL
181PLDSFEGWLL KWTNYLKGYQ RRWFVLGNGL LSYYRNQGEM AHTCRGTINL STAHIDTEDS
241CGILLTSGAR SYHLKASSEV DRQQWITALE LAKAKAVRVM NTHSDDSGDD DEATTPADKS
301ELHHTLKNLS LKLDDLSTCN DLIAKHGAAL QRSLTELDGL KIPSESGEKL KVVNERATLF
361RITSNAMINA CRDFLELAEI HSRKWQRALQ YEQEQRVHLE ETIEQLAKQH NSLERAFHSA
421PGRPANPSKS FIEGSLLTPK GEDSEEDEDT EYFDAMEDST SFITVITEAK EDRKAEGSTG
481TSSVDWSSAD NVLDGASLVP KGSSKVKRRV RIPNKPNYSL NLWSIMKNCI GRELSRIPMP
541VNFNEPLSML QRLTEDLEYH HLLDKAVHCT SSVEQMCLVA AFSVSSYSTT VHRIAKPFNP
601MLGETFELDR LDDMGLRSLC EQVSHHPPSA AHYVFSKHGW SLWQEITISS KFRGKYISIM
661PLGAIHLEFQ ASGNHYVWRK STSTVHNIIV GKLWIDQSGD IEIVNHKTND RCQLKFLPYS
721YFSKEAARKV TGVVSDSQGK AHYVLSGSWD EQMECSKVMH SSPSSPSSDG KQKTVYQTLS
781AKLLWKKYPL PENAENMYYF SELALTLNEH EEGVAPTDSR LRPDQRLMEK GRWDEANTEK
841QRLEEKQRLS RRRRLEACGP GSSCSSEEEK EADAYTPLWF EKRLDPLTGE MACVYKGGYW
901EAKEKQDWHM CPNIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

915 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.