Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ORC4L / ORC4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130543-100
Catalog Number: LS-G130543
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ORC4L(ORC4) (NM_001190881), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ORC4 | ORC4P | ORC4L | HsORC4
- Origin Species
- Human
- NCBI GenBank
- NM_002552
- SwissProt
- O43929
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEIEEVSENV LQVHLNGLLQ INDKIALKEI TRQLNLENVV GDKVFGSFAE NLSFLLEALK61KGDRTSSCPV IFILDEFDLF AHHKNQTLLY NLFDISQSAQ TPIAVIGLTC RLDILELLEK121RVKSRFSHRQ IHLMNSFGFP QYVKIFKEQL SLPAEFPDKV FAEKWNENVQ YLSEDRSVQE181VLQKHFNISK NLRSLHMLLM LALNRVTASH PFMTAVDLME ASQLCSMDSK ANIVHGLSVL241EICLIIAMKH LNDIYEEEPF NFQMVYNEFQ KFVQRKAHSV YNFEKPVVMK AFEHLQQLEL301IKPMERTSGN SQREYQLMKL LLDNTQIMNA LQKYPNCPTD VRQWATSSLS WLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
352 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.