Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ORC3L / ORC3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114487-100
Catalog Number: LS-G114487
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ORC3L(ORC3) (NM_012381), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LATHEO | Homolog of latheo | Drosophila | ORC3 | ORC3L | IMAGE50150
- Origin Species
- Human
- NCBI GenBank
- NM_012381
- SwissProt
- Q9UBD5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATSSMSKGC FVFKPNSKKR KISLPIEDYF NKGKNEPEDS KLRFETYQLI WQQMKSENER61LQEELNKNLF DNLIEFLQKS HSGFQKNSRD LGGQIKLREI PTAALVLGVN VTDHDLTFGS121LTEALQNNVT PYVVSLQAKD CPDMKHFLQK LISQLMDCCV DIKSKEEESV HVTQRKTHYS181MDSLSSWYMT VTQKTDPKML SKKRTTSSQW QSPPVVVILK DMESFATKVL QDFIIISSQH241LHEFPLILIF GIATSPIIIH RLLPHAVSSL LCIELFQSLS CKEHLTTVLD KLLLTTQFPF301KINEKVLQVL TNIFLYHDFS VQNFIKGLQL SLLEHFYSQP LSVLCCNLPE AKRRINFLSN361NQCENIRRLP SFRRYVEKQA SEKQVALLTN ERYLKEETQL LLENLHVYHM NYFLVLRCLH421KFTSSLPKYP LGRQIRELYC TCLEKNIWDS EEYASVLQLL RMLAKDELMT ILEKCFKVFK481SYCENHLGST AKRIEEFLAQ FQSLDETKEE EDASGSQPKG LQKTDLYHLQ KSLLEMKELR541RSKKQTKFEV LRENVVNFID CLVREYLLPP ETQPLHEVVY FSAAHALREH LNAAPRIALH601TALNNPYYYL KNEALKSEEG CIPNIAPDIC IAYKLHLECS RLINLVDWSE AFATVVTAAE661KMDANSATSE EMNEIIHARF IRAVSELELL GFIKPTKQKT DHVARLTWGG CTRTRPLEQKLISEEDLAANDILDYKDDDDKV
711 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.