Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ORC2L / ORC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115474-100
Catalog Number: LS-G115474
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ORC2 (NM_006190), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ORC2L | ORC2
- Origin Species
- Human
- NCBI GenBank
- NM_006190
- SwissProt
- Q13416
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSKPELKEDK MLEVHFVGDD DVLNHILDRE GGAKLKKERA QLLVNPKKII KKPEYDLEED61DQEVLKDQNY VEIMGRDVQE SLKNGSATGG GNKVYSFQNR KHSEKMAKLA SELAKTPQKS121VSFSLKNDPE ITINVPQSSK GHSASDKVQP KNNDKSEFLS TAPRSLRKRL IVPRSHSDSE181SEYSASNSED DEGVAQEHEE DTNAVIFSQK IQAQNRVVSA PVGKETPSKR MKRDKTSDLV241EEYFEAHSSS KVLTSDRTLQ KLKRAKLDQQ TLRNLLSKVS PSFSAELKQL NQQYEKLFHK301WMLQLHLGFN IVLYGLGSKR DLLERFRTTM LQDSIHVVIN GFFPGISVKS VLNSITEEVL361DHMGTFRSIL DQLDWIVNKF KEDSSLELFL LIHNLDSQML RGEKSQQIIG QLSSLHNIYL421IASIDHLNAP LMWDHAKQSL FNWLWYETTT YSPYTEETSY ENSLLVKQSG SLPLSSLTHV481LRSLTPNARG IFRLLIKYQL DNQDNPSYIG LSFQDFYQQC REAFLVNSDL TLRAQLTEFR541DHKLIRTKKG TDGVEYLLIP VDNGTLTDFL EKEEEEATRT RPLEQKLISE EDLAANDILD601YKDDDDKV
608 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.