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OR10Z1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OR10Z1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G122274-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OR10Z1 (NM_001004478), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
OR10Z1 | Olfactory receptor 10Z1 | Olfactory receptor OR1-15 | OR1-15
Origin Species
Human
SwissProt
Q8NGY1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
34.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MGQTNVTSWR DFVFLGFSSS GELQLLLFAL FLSLYLVTLT SNVFIIIAIR LDSHLHTPMY
61LFLSFLSFSE TCYTLGIIPR MLSGLAGGDQ AISYVGCAAQ MFFSASWACT NCFLLAAMGF
121DRYVAICAPL HYASHMNPTL CAQLVITSFL TGYLFGLGMT LVIFHLSFCS SHEIQHFFCD
181TPPVLSLACG DTGPSELRIF ILSLLVLLVS FFFITISYAY ILAAILRIPS AEGQKKAFST
241CASHLTVVII HYGCASFVYL RPKASYSLER DQLIAMTYTV VTPLLNPIVY SLRNRAIQTA
301LRNAFRGRLL GKG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

313 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.