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OR10H1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OR10H1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114258-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OR10H1 (NM_013940), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Olfactory receptor 10H1 | OR10H1 | Olfactory receptor OR19-27
Origin Species
Human
NCBI GenBank
NM_013940
SwissProt
Q9Y4A9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
35.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MQRANHSTVT QFILVGFSVF PHLQLMLFLL FLLMYLFTLL GNLLIMATVW SERSLHTPMY
61LFLCALSVSE ILYTVAIIPR MLADLLSTQR SIAFLACASQ MFFSFSFGFT HSFLLTVMGY
121DRYVAICHPL RYNVLMSPRG CACLVGCSWA GGLVMGMVVT SAIFHLAFCG HKEIHHFACH
181VPPLLKLACG DDVLVVAKGV GLVCITALLG CFLLILLSYA FIVAAILKIP SAEGRNKAFS
241TCASHLTVVV VHYGFASVIY LKPKSPQSLE GDTLMGITYT VLTPFLSPII FSLRNKELKV
301AMKKTFFSKL YPEKNVMM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

318 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.