Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
OPN4 / Melanopsin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120783-100
Catalog Number: LS-G120783
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Melanopsin(OPN4) (NM_001030015), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Opsin 4 (melanopsin) | Melanopsin | MOP | OPN4 | Opsin 4 | Opsin-4
- Origin Species
- Human
- NCBI GenBank
- AF147788
- SwissProt
- Q9UHM6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPPSGPRVP PSPTQEPSCM ATPAPPSWWD SSQSSISSLG RLPSISPTAP GTWAAAWVPL61PTVDVPDHAH YTLGTVILLV GLTGMLGNLT VIYTFCRAVL RGVTVMMQSR SLRTPANMFI121INLAVSDFLM SFTQAPVFFT SSLYKQWLFG ETGCEFYAFC GALFGISSMI TLTAIALDRY181LVITRPLATF GVASKRRAAF VLLGVWLYAL AWSLPPFFGW SAYVPEGLLT SCSWDYMSFT241PAVRAYTMLL CCFVFFLPLL IIIYCYIFIF RAIRETGRAL QTFGACKGNG ESLWQRQRLQ301SECKMAKIML LVILLFVLSW APYSAVALVA FAGYAHVLTP YMSSVPAVIA KASAIHNPII361YAITHPKYRV AIAQHLPCLG VLLGVSRRHS RPYPSYRSTH RSTLTSHTSN LSWISIRRRQ421ESLGSESEVG WTHMEAAAVW GAAQQANGRS LYGQGLEDLE AKAPPRPQGH EAETPGKTKG481LIPSQDPRMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
489 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.