Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
OPCML / OBCAM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99274-100
Catalog Number: LS-G99274
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OPCML (NM_001012393), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IGLON1 | OBCAM | OPCM | OPCML | IgLON family member 1
- NCBI GenBank
- NM_002545
- SwissProt
- Q14982
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.27 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYHPAYWVVF SATTALLFIP GVPVRSGDAT FPKAMDNVTV RQGESATLRC TIDDRVTRVA61WLNRSTILYA GNDKWSIDPR VIILVNTPTQ YSIMIQNVDV YDEGPYTCSV QTDNHPKTSR121VHLIVQVPPQ IMNISSDITV NEGSSVTLLC LAIGRPEPTV TWRHLSVKEG QGFVSEDEYL181EISDIKRDQS GEYECSALND VAAPDVRKVK ITVNYPPYIS KAKNTGVSVG QKGILSCEAS241AVPMAEFQWF KEETRLATGL DGMRIENKGR MSTLTFFNVS EKDYGNYTCV ATNKLGNTNA301SITLYGPGAV IDGVNSASRA LACLWLSGTL LAHFFIKFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
338 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.