Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ONECUT3 / OC3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119646-100
Catalog Number: LS-G119646
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ONECUT3 (NM_001080488), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OC-3 | OC3 | One cut homeobox 3 | One cut domain family member 3 | Transcription factor ONECUT-3 | ONECUT3
- Origin Species
- Human
- NCBI GenBank
- NM_001080488
- SwissProt
- O60422
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELSLESLGG LHSVAHAQAG ELLSPGHARS AAAQHRGLVA PGRPGLVAGM ASLLDGGGGG61GGGGAGGAGG AGSAGGGADF RGELAGPLHP AMGMACEAPG LGGTYTTLTP LQHLPPLAAV121ADKFHQHAAA AAVAGAHGGH PHAHPHPAAA PPPPPPPQRL AASVSGSFTL MRDERAALAS181VGHLYGPYGK ELPAMGSPLS PLPNALPPAL HGAPQPPPPP PPPPLAAYGP PGHLAGDKLL241PPAAFEPHAA LLGRAEDALA RGLPGGGGGT GSGGAGSGSA AGLLAPLGGL AAAGAHGPHG301GGGGPGGSGG GPSAGAAAEE INTKEVAQRI TAELKRYSIP QAIFAQRILC RSQGTLSDLL361RNPKPWSKLK SGRETFRRMW KWLQEPEFQR MSALRLAACK RKEQEQQKER ALQPKKQRLV421FTDLQRRTLI AIFKENKRPS KEMQVTISQQ LGLELNTVSN FFMNARRRCM NRWAEEPSTA481PGGPAGATAT FSKATRTRPLEQKLISEEDLAANDILDYKDDDDKV
494 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.