Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ONECUT2 / OC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116361-100
Catalog Number: LS-G116361
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ONECUT2 (NM_004852), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HNF-6-beta | HNF6B | OC-2 | OC2 | One cut homeobox 2 | One cut domain family member 2 | ONECUT2 | Onecut 2 | Transcription factor ONECUT-2
- Origin Species
- Human
- NCBI GenBank
- NM_004852
- SwissProt
- O95948
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKAAYTAYRC LTKDLEGCAM NPELTMESLG TLHGPAGGGS GGGGGGGGGG GGGGPGHEQE61LLASPSPHHA GRGAAGSLRG PPPPPTAHQE LGTAAAAAAA ASRSAMVTSM ASILDGGDYR121PELSIPLHHA MSMSCDSSPP GMGMSNTYTT LTPLQPLPPI STVSDKFHHP HPHHHPHHHH181HHHHQRLSGN VSGSFTLMRD ERGLPAMNNL YSPYKEMPGM SQSLSPLAAT PLGNGLGGLH241NAQQSLPNYG PPGHDKMLSP NFDAHHTAML TRGEQHLSRG LGTPPAAMMS HLNGLHHPGH301TQSHGPVLAP SRERPPSSSS GSQVATSGQL EEINTKEVAQ RITAELKRYS IPQAIFAQRV361LCRSQGTLSD LLRNPKPWSK LKSGRETFRR MWKWLQEPEF QRMSALRLAA CKRKEQEPNK421DRNNSQKKSR LVFTDLQRRT LFAIFKENKR PSKEMQITIS QQLGLELTTV SNFFMNARRR481SLEKWQDDLS TGGSSSTSST CTKATRTRPLEQKLISEEDLAANDILDYKDDDDKV
504 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.