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OLFML2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OLFML2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OLFML2B antibodies →
$504.00each
Catalog Number: LS-G113211-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OLFML2B (NM_015441), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP586L151 | Photomedin-2 | RP11-227F8.1 | Olfactomedin-like 2B | Olfactomedin-like protein 2B | OLFML2B
Origin Species
Human
NCBI GenBank
NM_015441
SwissProt
Q68BL8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKPRLLVLY FALIVVPAWV SSIVLTGTSE PPDAQTVAPA EDETLQNEAD NQENVLSQLL
61GDYDKVKAMS EGSDCQCKCV VRPLGRDACQ RINAGASRKE DFYTVETITS GSSCKCACVA
121PPSALNPCEG DFRLQKLREA DSQDLKLSTI IDMLEGAFYG LDLLKLHSVT TKLVGRVDKL
181EEEVSKNLTK ENEQIKEDME EIRTEMNKRG KENCSENILD SMPDIRSALQ RDAAAAYAHP
241EYEERFLQEE TVSQQINSIE LLQTRPLALP EVVKSQRPLQ RQVHLRGRPA SQPTVIRGIT
301YYKAKVSEEE NDIEEQQDEF FSGDNGVDLL IEDQLLRHNG LMTSVTRRPA ATRQGHSTAV
361TSDLNARTAP WSSALPQPST SDPSIANHAS VGPTLQTTSV SPDPTRESVL QPSPQVPATT
421VAHTATQQPA APAPPAVSPR EALMEAMHTV PVPPTTVRTD SLGKDAPAGW GTTPASPTLS
481PEEEDDIRNV IGRCKDTLST ITGPTTQNTY GRNEGAWMKD PLAKDERIYV TNYYYGNTLV
541EFRNLENFKQ GRWSNSYKLP YSWIGTGHVV YNGAFYYNRA FTRNIIKYDL KQRYVAAWAM
601LHDVAYEEAT PWRWQGHSDV DFAVDENGLW LIYPALDDEG FSQEVIVLSK LNAADLSTQK
661ETTWRTGLRR NFYGNCFVIC GVLYAVDSYN QRNANISYAF DTHTNTQIVP RLLFENEYSY
721TTQIDYNPKD RLLYAWDNGH QVTYHVIFAY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

750 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.