Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
OLFM2 / Olfactomedin-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102210-100
Catalog Number: LS-G102210
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OLFM2 (NM_058164), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NOE2 | Noelin-2 | NOELIN2 | NOELIN2_V1 | OLFM2 | Olfactomedin 2 | Olfactomedin-2 | Noelin 2 | OlfC
- Origin Species
- Human
- NCBI GenBank
- AF131839
- SwissProt
- O95897
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MWPLTVPPPL LLLLCSGLAG QTLFQNPEEG WQLYTSAQAP DGKCICTAVI PAQSTCSRDG61RSRELRQLME KVQNVSQSME VLELRTYRDL QYVRGMETLM RSLDARLRAA DGSLSAKSFQ121ELKDRMTELL PLSSVLEQYK ADTRTIVRLR EEVRNLSGSL AAIQEEMGAY GYEDLQQRVM181ALEARLHACA QKLGCGKLTG VSNPITVRAM GSRFGSWMTD TMAPSADSRV WYMDGYYKGR241RVLEFRTLGD FIKGQNFIQH LLPQPWAGTG HVVYNGSLFY NKYQSNVVVK YHFRSRSVLV301QRSLPGAGYN NTFPYSWGGF SDMDFMVDES GLWAVYTTNQ NAGNIVVSRL DPHTLEVMRS361WDTGYPKRSA GEAFMICGVL YVTNSHLAGA KVYFAYFTNT SSYEYTDVPF HNQYSHISML421DYNPRERALY TWNNGHQVLY NVTLFHVIST SGDPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
454 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.